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Keto-Test ™

Keto-Test ™ (Beta-hydroxybutyric acid)

Keto-Test is a simple cow-side milk test strip that provides dairy producers an easy to use, cost-effective ketosis-screening program to help identify subclinical ketosis (SCK) in their herd.
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Keto-Test ™ (Beta-hydroxybutyric acid)
Keto-Test ™ (Beta-hydroxybutyric acid)

Keto-Test™ is a simple cow-side milk test strip that provides dairy producers an easy to use, cost-effective ketosis screening program to help identify subclinical ketosis (SCK) in their herds.

Ketosis occurs in dairy cows as a consequence of the negative energy balance in the early postpartum period, and can result in lowered milk production, impaired fertility, and increased risk of periparturient diseases, such as displaced abomasum. Subclinical ketosis usually precedes clinical ketosis, and is much more common. Subclinical ketosis causes greater economic losses on a herd basis than clinical ketosis. Subclinical ketosis can be diagnosed by measuring ketone bodies present in blood, urine or milk. The Keto-Test (Ketolac test strip) is a convenient, very effective method of detecting cows with subclinical ketosis.12

Beta-hydroxybutyric acid in the milk passes through the reagent part of the test strip and is converted by beta-hydroxybutyric acid dehydrogenase (BHBDH) to acetoacetic acid (AcAc). The NADH produced from NAD in the process reduces nitrotetrazolium blue (NTB) to fomazan, which is purple in colour. The concentration of beta-hydroxybutyric acid in the milk can then be estimated from the change in colour.

Important Safety Information

Before using this product, please refer to the label for complete instructions.

Directions For Use

  1. Remove test strip container from the refrigerator and bring to room temperature. Immediately close the container again firmly.
  2. Dip a single test strip for 3 seconds into a small quantity of fresh milk (in a clean container). If the milk sample has been standing for some time it must be thoroughly shaken before carrying out the test. It, too, should be at room temperature.
  3. Shake twice vigorously to remove excess milk.
  4. After 1 minute read test strip colour and compare to colour chart on bottle label (if colour reaction varies at the margin, base the reading on the central colour).

This milk test strip system semi quantitatively measures Beta-hydroxybutyric acid (BHBA), which is a key ketone body present in milk. The concentration of BHBA present in the milk can be estimated by the strength of the colour change reaction. The more purple the result, the higher the concentration of ketones present in the milk and the greater the likelihood of ketosis. BHBA levels > 200μmol/l of milk were 4 times more likely to come from cows with subclinical ketosis. The Keto-Test was found to be the best cowside method for measuring BHBA.1, 2

Interpretation of results

Milk BHBA concentration Keto-Test™ Assessment
0-50μmol/l Normal (-)
100μmol/l Weak positive (+/-)
200μmol/l Positive (+)
>500μmol/l Highly positive (++)

1. Geishauser T, Leslie K, Tenhag J and Bashiri. Evaluation of Eight Cow-side Ketone Tests in Milk for Detection of Subclinical Ketosis in Dairy Cows. 2000. J. Dairy Sci. 83:296-299

2. Geishauser T, Leslie K, Kelton D and Duffield T. Evaluation of Five Cow-side Tests for Use with Milk to Detect Subclinical Ketosis in Dairy Cows. 1998. J.Dairy Sci. 81:438-443